Fabrication of novel poly(N-vinylcaprolactam)-coated UiO-66-NH2 metal organic framework nanocarrier for the controlled release of doxorubicin against A549 lung cancer cells

The nano metal-organic frameworks (NMOFs) have been developed for drug delivery systems due to their high porosity and large specific surface area. In this work, UiO-66-NH2 NMOFs were synthesized via the microwave heating method and doxorubicin (DOX) as an anticancer drug was incorporated into the UiO-66-NH2 NMOFs. Then, poly(N-vinylcaprolactam) (PNVCL) synthesized by the free radical polymerization was coated on the UiO66-NH2 NMOFs surface to fabricate dual pH/temperature-responsive nanocomposite against A549 lung cancer cells death in vitro. The synthesized nanocarriers were characterized using FTIR, H-1 NMR, DLS, XRD, SEM, FESEM, TGA, and BET analysis. The average particle sizes of UiO-66-NH2, PNVCL 1% and PNVCL 2%-coated UiO-66-NH2/DOX NMOFs were found to be 190 +/- 110 nm, 265 +/- 140 nm, and 360 +/- 150 nm, respectively. TGA analysis showed that the PNVCL percentages-coated UiO-66-NH2 NMOFs were found to be about 17.5%, and 27.3% for NMOFs incubated in 1% and 2% PNVCL solutions, respectively. The BET surface area of UiO-66-NH2 NMOFs, UiO-66-NH2 NMOFs/DOX 100 mu g mL(-1), and PNVCL 1%-coated NMOFs/DOX was found to be 1052, 121, and 87 m(2)g(-1), respectively. The DOX release data of UiO-66-NH2 and PNVCL-coated UiO-66-NH2/DOX were evaluated under pH values of 5.5, 7.4, and temperatures of 25 degrees C, 37 degrees C. The maximum cytotoxicity of A549 cancer cells treated with PNVCL 1%-coated UiO-66-NH2/DOX 100 mu g mL(-1) NMOFs was found to be 76%. The obtained results revealed the high capability of UiO-66-NH2/DOX/PNVCL dual-responsive nanocomposite for delivery of anticancer drugs.

Automated summary

Nano metal-organic frameworks (NMOFs), such as UiO-66-NH2, have been developed as drug delivery systems due to their high porosity and specific surface area, with PNVCL coating providing a dual pH/temperature-responsive nanocomposite against A549 lung cancer cells. Characterization studies including FTIR, H-1 NMR, DLS, XRD, SEM, FESEM, TGA, and BET analysis were conducted to evaluate the synthesized nanocarriers. The results indicated the significant potential of UiO-66-NH2/DOX/PNVCL dual-responsive nanocomposite for effective delivery of anticancer drugs.