4.7 Article

Nisin Z attenuates lipopolysaccharide-induced mastitis by inhibiting the ERK1/2 and p38 mitogen-activated protein kinase signaling pathways

期刊

JOURNAL OF DAIRY SCIENCE
卷 105, 期 4, 页码 3530-3543

出版社

ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2021-21356

关键词

nisin Z; mastitis; mitogen-activated protein kinase; blood-milk barrier

资金

  1. National Natural Science Foundation of China (Beijing, China) [U20A2066, 31972049]
  2. Strategic Priority Re-search Program of the Chinese Academy of Sciences (Beijing, China) [XDA26040201]

向作者/读者索取更多资源

Nisin Z has potential anti-inflammatory effects on mastitis by inhibiting the activation of the ERK1/2 and p38 MAPK signaling pathway and promoting the blood-milk barrier.
Nisin Z is a possible alternative for treating bovine mastitis by inhibiting mastitis-causing pathogens and having anti-inflammatory activity. However, the anti-inflammatory mechanism of nisin Z on mastitis is un-known. Our study aimed to investigate the mechanisms of nisin Z on mastitis. Our results showed that nisin Z inhibited the activation of the ERK1/2 and p38 mitogen-activated protein kinase (MAPK) signaling pathway, decreased the release of pro-inflammatory cy-tokines (i.e., tumor necrosis factor-alpha, IL-1 beta, and IL-6), and increased the anti-inflammatory cytokine (IL-10) in lipopolysaccharide (LPS)-induced MCF10A cells. After intraperitoneal injection, nisin Z significantly decreased inflammatory cell infiltration in the mam-mary gland, as well as decreased myeloperoxidase and pro-inflammatory cytokines in serum and mammary gland. Western blot analysis revealed that nisin Z also dramatically suppressed the activation of the ERK1/2 and p38 MAPK signaling pathways in LPS-induced mastitis mice. We also found that nisin Z treatment could enhance the blood-milk barrier. In summary, our study demonstrated that nisin Z exerted an anti-inflammatory effect by inhibiting the ERK1/2 and p38 MAPK signaling pathway and promoting the blood-milk barrier on LPS-induced mastitis.

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