期刊
JOURNAL OF DAIRY SCIENCE
卷 105, 期 4, 页码 3530-3543出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2021-21356
关键词
nisin Z; mastitis; mitogen-activated protein kinase; blood-milk barrier
资金
- National Natural Science Foundation of China (Beijing, China) [U20A2066, 31972049]
- Strategic Priority Re-search Program of the Chinese Academy of Sciences (Beijing, China) [XDA26040201]
Nisin Z has potential anti-inflammatory effects on mastitis by inhibiting the activation of the ERK1/2 and p38 MAPK signaling pathway and promoting the blood-milk barrier.
Nisin Z is a possible alternative for treating bovine mastitis by inhibiting mastitis-causing pathogens and having anti-inflammatory activity. However, the anti-inflammatory mechanism of nisin Z on mastitis is un-known. Our study aimed to investigate the mechanisms of nisin Z on mastitis. Our results showed that nisin Z inhibited the activation of the ERK1/2 and p38 mitogen-activated protein kinase (MAPK) signaling pathway, decreased the release of pro-inflammatory cy-tokines (i.e., tumor necrosis factor-alpha, IL-1 beta, and IL-6), and increased the anti-inflammatory cytokine (IL-10) in lipopolysaccharide (LPS)-induced MCF10A cells. After intraperitoneal injection, nisin Z significantly decreased inflammatory cell infiltration in the mam-mary gland, as well as decreased myeloperoxidase and pro-inflammatory cytokines in serum and mammary gland. Western blot analysis revealed that nisin Z also dramatically suppressed the activation of the ERK1/2 and p38 MAPK signaling pathways in LPS-induced mastitis mice. We also found that nisin Z treatment could enhance the blood-milk barrier. In summary, our study demonstrated that nisin Z exerted an anti-inflammatory effect by inhibiting the ERK1/2 and p38 MAPK signaling pathway and promoting the blood-milk barrier on LPS-induced mastitis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据