期刊
JOURNAL OF CONTROLLED RELEASE
卷 338, 期 -, 页码 505-526出版社
ELSEVIER
DOI: 10.1016/j.jconrel.2021.08.038
关键词
Extracellular vesicles; Microvesicles; Exosomes; Mitochondrial transfer; Mitochondrial function; BBB protection; Ischemic stroke
资金
- Duquesne University (DU)
- Faculty Development Fund (Office of Research, DU)
- Neurodegenerative Undergraduate Research Experience (NURE) [R25NS100118]
- [P30CA047904]
It was shown for the first time that microvesicles derived from human brain endothelial cells can transfer polarized mitochondria to recipient cells and increase ATP levels. The study also revealed a high association to glycolysis-related processes and demonstrated the selective transfer of mitochondria to increase endothelial cell survival under ischemic conditions.
We have demonstrated, for the first time that microvesicles, a sub-type of extracellular vesicles (EVs) derived from hCMEC/D3: a human brain endothelial cell (BEC) line transfer polarized mitochondria to recipient BECs in culture and to neurons in mice acute brain cortical and hippocampal slices. This mitochondrial transfer increased ATP levels by 100 to 200-fold (relative to untreated cells) in the recipient BECs exposed to oxygen-glucose deprivation, an in vitro model of cerebral ischemia. We have also demonstrated that transfer of microvesicles, the larger EV fraction, but not exosomes resulted in increased mitochondrial function in hypoxic endothelial cultures. Gene ontology and pathway enrichment analysis of EVs revealed a very high association to glycolysisrelated processes. In comparison to heterotypic macrophage-derived EVs, BEC-derived EVs demonstrated a greater selectivity to transfer mitochondria and increase endothelial cell survival under ischemic conditions.
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