期刊
JOURNAL OF CLINICAL LABORATORY ANALYSIS
卷 36, 期 2, 页码 -出版社
WILEY
DOI: 10.1002/jcla.24194
关键词
biomarkers; microRNA; nasopharyngeal carcinoma
资金
- Science and Technology Development Fund Project of Shenzhen [JCYJ20180507183102747]
- Shenzhen High-level Hospital Construction Fund, Basic Research Project of Peking University Shenzhen Hospital [JCYJ2017001, JCYJ2017004, JCYJ2017005, JCYJ2017006, JCYJ2017007, JCYJ2017012]
- Clinical Research Project of Peking University Shenzhen Hospital [LCYJ2017001]
- Clinical Research Project of Shenzhen Health Commission [SZLY2018023]
MicroRNAs are stable in serum and specific to different tumor types, making them a potential non-invasive biomarker for cancer detection. A three-miRNA panel has been shown to have high diagnostic efficiency for nasopharyngeal carcinoma. Further analysis suggests potential target genes for this miRNA panel.
Background Nasopharyngeal carcinoma is cancer with unique epidemiological characteristics, showing obvious ethnicity, gender, and geographical prevalence. More and more evidence shows that microRNAs are stable in serum and are specific to different tumor types. Therefore, miRNA is a new non-invasive biomarker for cancer detection. Methods The experiment is divided into three stages, namely, the screening stage, the training stage, and the verification stage. We took 54 patients with nasopharyngeal carcinoma and 108 healthy controls as the research objects. We use the receiver-operating characteristic (ROC) curve and area under the ROC curve (AUC) to evaluate the diagnostic value of miRNA. Finally, a three-miRNA panel with high diagnostic efficiency was constructed. In addition, we conducted biological information analysis of these miRNAs to explore their functions. Results In NPC patients, the expression of five serum miRNAs (miR-29c-3p, miR-143-5p, miR-150-5p, miR-145-3p, and miR-205-5p) is significantly dysregulated. Among them, the diagnostic value of these three miRNAs (miR-29c-3p, AUC = 0.702; miR-143-5p, AUC = 0.733; and miR-205-5p, AUC = 787) is more prominent. The diagnostic panel constructed by them has a higher diagnostic value (AUC = 0.902). Through the analysis of the TCGA data set, the target gene of the three-miRNA panel may be KLF7, NRG1, SH3BGRL2, and SYNPO2. Conclusion The three-miRNA panel (miR-29c-3p, miR-143-5p, and miR-205-5p) may become a novel non-invasive biological marker for nasopharyngeal cancer screening.
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