期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1665, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.chroma.2022.462828
关键词
Veterinary drugs and other contaminants; UHPLC-MS/MS; Raw honey
资金
- basic Public Welfare Research Program of Zhejiang Province [LGC22C1900 01]
- Ningbo Science and Technology Innovation Team [2015C110018]
- Major technological innovation project of Ningbo National High-tech Zone [20181CX050016]
- Ningbo Science and Technology Bureau [202002N30B6B6]
A generic, rapid, simple, and low-cost analytical method for screening and confirming 400 veterinary drugs and other contaminants in raw honey was developed. The method employs one-step extraction and ultra-high performance liquid chromatography coupled with electrospray ionization and tandem mass spectrometry, providing good extraction efficiency and analysis accuracy.
A generic, rapid, simple and low-cost analytical method for simultaneously screening and confirming 400 veterinary drugs and other contaminants in raw honey was developed. The method was based on onestep extraction and ultra-high performance liquid chromatography coupled with electrospray ionization and tandem mass spectrometry. A well-designed extraction method results in the complete precipitation of proteins and separation of analytes from carbohydrates and reduces the time and cost of analysis by covering polar to non-polar analytes. Optimized pretreatment processes lead to no significant interference on analysis from complicated sample matrix. Competent linearity was found for all of target compounds with linear regression coefficients (R) higher than 0.99. Detection limits ranged from 0.05 mu g/kg to 10 mu g/kg. Average recovery rates spiked in raw honey and ranged from 57.3% to 139.8%, and associated relative standard deviation values ranged from 0.4% to 25.2% under selected conditions. The extraction sensitivity, linearity, recovery, and precision of the method were validated. The results clearly demonstrated the feasibility of using this approach in raw honey purchase. The application of this method, which improved efficiency and the coverage of residues, may considerably reduce effort and time in routine monitoring programs. (C) 2022 Published by Elsevier B.V.
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