A Polymer-based Monolithic Capillary Column with Polymyxin-B Chiral Selector for the Enantioselective Nano-High Performance Liquid Chromatographic Pharmaceutical Analysis * , **

Herein, we report the first use of Polymyxin-B antibiotic as a enantio-selector in polymer monolithic capillary. The capillaries were functionalised, characterized and tested for the enantioselective nanoHPLC separation of 50 racemic pharmaceutical drugs. They have been easily prepared by immobilizing Polymyxin-B over the organic polymer for 48 h (P1) or encapsulating Polymyxin-B within the organic polymer (P2) and tested for the enantioselective resolution of racemic drugs. Acceptable resolution was achieved for 21 drugs using RP-HPLC conditions on both (P1) and (P2) capillary columns, while no separation was observed under NP-HPLC conditions. Polymyxin-B is commercially available, easily solubilized and stable in both acidic and neutral media. The developed Polymyxin-B-based polymer monolithic capillaries provide a promising expansion of platform in enantioselective HPLC separations. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

This study reports the first use of Polymyxin-B antibiotic as an enantio-selector in polymer monolithic capillary for the enantioselective nanoHPLC separation of 50 racemic pharmaceutical drugs. Polymyxin-B was immobilized or encapsulated within the organic polymer, leading to acceptable resolution of 21 drugs under RP-HPLC conditions. This development represents a promising expansion of platform in enantioselective HPLC separations.