期刊
JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷 61, 期 1, 页码 15-31出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/chromsci/bmac002
关键词
UPLC-ESI-QTOF-MS; MS; Chaenomeles speciosa (Sweet) Nakai; phenolic compounds; terpenoids; organic acids; alkaloids; amino acids; Anti-inflammatory; NO inhibition
资金
- Guangdong Province Special Funds for Science and Technology of China [[2019] 113-66]
- Guangdong collaborative innovation and environment construction Foundation of China [2016B090918129]
- Shantou Science and Technology Project [[2018] 155-11, [2019] 775]
This study used an advanced analytical method to investigate the phytochemical composition of Chaenomeles speciosa fruit and evaluate its anti-inflammatory activity. The results revealed a wide range of phytochemicals in the fruit, which have potential therapeutic effects for various diseases.
Chaenomeles speciosa (Sweet) Nakai (C. speciosa Nakai) is a popular fruit widely used in China for its health-promoting properties. The presences of phytochemical compositions in the plants play an important role in the health benefits. Nevertheless, the detailed information of these ingredients is still unknown. Therefore, in this work, an untargeted analytical method based on ultra-high-performance liquid chromatography-quadrupole-time of flight coupled to mass spectrometry in two different ionization modes was used to qualitative the phytochemicals in C. speciosa Nakai, meanwhile, the anti-inflammatory activity of these phytochemicals was researched through detecting the inhibition of nitric oxide (NO) that was induced by lipopolysaccharide in RAW 264.7 murine macrophage cells. The results showed that there were totally 175 primary and secondary metabolites were identified in the fruit of C. speciosa Nakai, including phenols, terpenoids, flavonoids and other phyto-constituents. Actually, most compounds were described in C. speciosa Nakai fruits for the first time. Besides, the anti-inflammatory activity was measured by the result of NO inhibition rate, the consequence showed that the value of half-inhibitory concentration (IC50) was 365.208 mu g/mL. These results indicate that C. speciosa Nakai is an efficient medicinal fruit, which owns various bioactivities and has the potential to treat various diseases.
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