期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 26, 期 8, 页码 2205-2217出版社
WILEY
DOI: 10.1111/jcmm.17242
关键词
apoptosis; cancer; ER stress; lncRNA; resveratrol
资金
- National Natural Science Foundation of China [81502582]
- Fundamental Research Funds for the Central Universities [N182004002]
- Natural Science Foundation of Liaoning Province [2021-MS--104]
- Fundamental Scientific Research Fund of Liaoning Provincial Education Department [LJKQZ2021002]
Resveratrol inhibits the survival of gastric tumor cells by promoting apoptosis, autophagy, and endoplasmic reticulum stress. It also alters the expression of several long non-coding RNAs, and knockdown of H19 enhances the therapeutic effect of resveratrol.
The phytoalexin resveratrol exhibits anti-tumour activity in many types of cancer. In this study, we showed that resveratrol suppressed the survival of gastric tumour cells both in vivo and in vitro. Resveratrol promoted apoptosis, autophagy and endoplasmic reticulum (ER) stress in a dose-dependent manner. RNA-seq analysis showed that multiple cell death signalling pathways were activated after resveratrol treatment, while the use of ER stress activators (tunicamycin and thapsigargin) in combinatorial with resveratrol led to further inhibition of cancer cell survival. Results also showed that resveratrol altered the expression of several long non-coding RNAs (lncRNAs), including MEG3, PTTG3P, GAS5, BISPR, MALAT1 and H19. Knockdown of H19 in resveratrol-treated cells further enhanced the effects of resveratrol on apoptosis, ER stress and cell cycle S-phase arrest. Furthermore, the migratory ability of resveratrol-treated cells was dramatically decreased after H19 knockdown. In conclusion, resveratrol inhibited cancer cell survival, while knockdown of lncRNA H19 resulted in increased sensitivity to resveratrol therapy.
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