期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 26, 期 1, 页码 108-122出版社
WILEY
DOI: 10.1111/jcmm.17053
关键词
AKT pathway; apoptosis; ER stress; lung cancer; proliferation; VLX1570
资金
- Jiangsu Provincial Key Medical Discipline [ZDXKA2016009]
- Postdoctoral Science Foundation of China [2020M671543]
- Postdoctoral Research Foundation of Jiangsu Province [2020Z009]
The study identified VLX1570 as a potent inhibitor for lung cancer, capable of inhibiting cell proliferation by regulating the cell cycle and inducing apoptosis. Furthermore, VLX1570 activates the PERK/IRE1/ATF6 pathway, inducing ER stress and ultimately leading to apoptosis in lung cancer cells.
The ubiquitin-proteasome system (UPS) possesses unique functions in tumorigenesis and has great potential for targeting tumours. The effectiveness of inhibitors targeting UPS in solid tumours remains to be studied. We screened a library of inhibitors targeting the ubiquitination system and found the highly potent, low-concentration inhibitor molecule VLX1570 that specifically killed lung cancer cells. VLX1570 is an inhibitor of deubiquitinating enzyme activity and has also shown potential for preclinical cancer treatment. We found that VLX1570 significantly inhibited lung cancer cells proliferation and colony formation. VLX1570 induced a G2/M cell cycle arrest by downregulating CDK1 and CyclinB1. Moreover, VLX1570 significantly promoted the mitochondrial-associated apoptosis. Mechanistically speaking, VLX1570 activated the PERK/IRE1/ATF6 pathway and induced ER stress in lung cancer cell lines. The inhibition of ER stress by tauroursodeoxycholic acid sodium or 4-phenylbutyric acid enhanced VLX1570-induced apoptosis. In addition, VLX1570 treatment led to the inactivation of Akt signalling and inhibited the proliferation of lung cancer cells by downregulating the Akt pathway. Moreover, combined treatment with VLX1570 and Afatinib or Gefitinib induced synergistic anti-lung cancer activity. Our present study demonstrated a novel therapy using VLX1570, which inhibited the proliferation and increased apoptosis in human lung cancer.
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