期刊
JOURNAL OF CELL SCIENCE
卷 135, 期 1, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.259191
关键词
Heart; Intron retention; Ion channels; Minor spliceosome; Splicing
类别
资金
- Rembrandt Institute of Cardiovascular Science
- Netherlands Research Initiative [CVON ARENA PRIME] [CVON2018-30 PREDICT2, CVON2015-12 e-Detect]
- Fondation pour la Recherche Me'dicale [PBR201810007613]
- University of Amsterdam
The study reveals that the minor spliceosome regulates the electrophysiological properties of cardiomyocytes by modulating the expression and function of voltage-dependent ion channels. This finding has important implications for genetic diseases and acquired diseases such as heart failure, in which there are changes in minor spliceosome activity.
Eukaryotic genomes contain a tiny subset of 'minor class' introns with unique sequence elements that require their own splicing machinery. These minor introns are present in certain gene families with specific functions, such as voltage-gated Na+ and voltage-gated Ca2+ channels. Removal of minor introns by the minor spliceosome has been proposed as a post-transcriptional regulatory layer, which remains unexplored in the heart. Here, we investigate whether the minor spliceosome regulates electrophysiological properties of cardiomyocytes by knocking down the essential minor spliceosome small nuclear snRNA component U6atac in neonatal rat ventricular myocytes. Loss of U6atac led to robust minor intron retention within Scn5a and Cacna1c, resulting in reduced protein levels of Na(v)1.5 and Ca(v)1.2 channels. Functional consequences were studied through patch-clamp analysis, and revealed reduced Na+ and L-type Ca2+ currents after loss of U6atac. In conclusion, minor intron splicing modulates voltage-dependent ion channel expression and function in cardiomyocytes. This may be of particular relevance in situations in which minor splicing activity changes, such as in genetic diseases affecting minor spliceosome components, or in acquired diseases in which minor spliceosome components are dysregulated, such as heart failure.
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