期刊
JOURNAL OF CARDIOTHORACIC SURGERY
卷 16, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13019-021-01701-w
关键词
Non-small cell lung cancer; circMMP11; miR-143; Methylation
The study found that circMMP11 is overexpressed in NSCLC and predicts poor survival, showing a close correlation with miR-143. In NSCLC cells, overexpression of circMMP11 reduced miR-143 expression and increased miR-143 methylation. MiR-143 reversed the enhancing effects of circMMP11 overexpression on cell proliferation.
Background CircMMP11 is a characterized circRNA with oncogenic function in breast cancer. In this study, we explored the involvement of circMMP11 in non-small cell lung cancer (NSCLC). Methods Paired cancer and non-cancer tissues were collected from 66 NSCLC patients, and the expression of circMMP11 and miR-143 in these tissues were detected using RT-qPCRs. Overexpression levels of circMMP11 and miR-143 were performed by transfection, and their crosstalk was analyzed by RT-qPCRs. The effect of circMMP11 overexpression on miR-143 methylation was analyzed by methylation-specific PCR. CCK-8 assay was performed to analyze the roles of miR-143 and circMMP11 in regulating NSCLC cell proliferation. Results We found that circMMP11 was overexpressed in NSCLC and predicted patients' poor survival. Moreover, a close correlation between circMMP11 and miR143 was observed. In NSCLC cells, circMMP11 overexpression reduced miR-143 expression and increased miR-143 methylation. CCK-8 assay analysis showed that miR-143 reversed the enhancing effects of circMMP11 overexpression on cell proliferation. Conclusions CircMMP11 is overexpressed in NSCLC and predicts poor survival. In addition, circMMP11 may downregulate miR-143 through methylation to suppress cell proliferation.
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