期刊
JOURNAL OF BIOPHOTONICS
卷 15, 期 5, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.202100319
关键词
bacterial inactivation; Custodiol; kidney decontamination; kidney transplantation; ultraviolet-c radiation
资金
- CAPES [88887.351897/2019-00]
- CNPq
- FAPESP [CEPOF 2013/07276-1]
- FundacAo de Apoio a Fisica e a Quimica (FAFQ)
- CAPES
This article demonstrates the feasibility of reducing bacterial load in a kidney model by using ultraviolet-C as a germicidal agent. The results show that the bacterial load in the perfusate can be fully eliminated in less than 30 minutes of liquid circulation and irradiation. A modeling approach is used to verify the decrease in bacterial load when an organ is present in the circuit.
Organ transplantations have an increasing medical relevance. It is becoming a regular procedure with an increase in individuals waiting for organs. The increase in the number of discarded organs is mostly due to the donor bacterial and/or viral infection. In this article, we are demonstrating the feasibility of reduction of the bacterial load in kidney model by using ultraviolet-C as a germicidal agent in circulating liquids. Using Staphylococcus aureus as a bacteria model, we were able to demonstrate that in less than 30 min of liquid circulation and associated to irradiation, the bacterial load of the perfusate Custodiol HTK, histidine-tryptophan-ketoglutarate (solution with 5 log CFU mL(-1)), was fully eliminated. A modeling approach was created to verify the possibility of bacterial load decrease, when an organ (here, a renal experimental model) is present in the circuit, releasing a varied rate of microorganisms over time, while the solution is irradiated. Finally, we use an ex vivo model with swine kidney, circulating in the preservation solution with a Lifeport Kidney Transporter machine, to demonstrate that we can contaminate the organ and then promote the elimination of the microbiological load. The results show the feasibility of the technique.
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