4.7 Article

A new bio-active asymmetric-Schiff base: synthesis and evaluation of calf thymus DNA interaction, topoisomerase IIα inhibition, in vitro antiproliferative activity, SEM analysis and molecular docking studies

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JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
卷 41, 期 7, 页码 2804-2822

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TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2022.2039297

关键词

Schiff bases; CT-DNA; topoisomerase II; molecular docking; antiproliferative activity; cytokinesis-block micronucleus assay; scanning electron microscopy

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In this study, a newly synthesized asymmetric-Schiff base was investigated for its interaction with DNA and its antiproliferative activity against tumor cells. The compound was found to effectively bind to DNA and exhibited a concentration- and time-dependent decrease in cell viability. It also induced DNA damage in tumor cells and showed potential as a DNA-targeting antitumor drug.
In this paper, the asymmetric-Schiff base 2-(4-(2-hydroxybenzylideneamino)benzylideneamino)benzoic acid (SB-2) was newly synthesized and characterized by various spectroscopic methods. The interaction of SB-2 with calf thymus DNA was investigated by UV-vis, fluorescence spectroscopy and molecular docking methods. It was determined that SB-2 effectively binds to DNA via the intercalation mode. DNA electrophoretic mobility experiments displayed that topoisomerase II alpha could not cleave pBR322 plasmid DNA in the presence of SB-2, confirming that the Schiff base acts as a topo II suppressor. In the molecular docking studies, SB-2 was found to show an affinity for both the DNA-topoisomerase II alpha complex and the DNA. In vitro antiproliferative activity of SB-2 was screened against HT-29 (colorectal) and HeLa (cervical) human tumor cell lines by MTT assay. SB-2 diminished the cell viability in a concentration- and incubation time-dependent manner. The ability of SB-2 to measure DNA damage in tumor cells was evaluated with cytokinesis-block micronucleus assay after incubation 24 h and 48 h. Light and scanning electron microscopy experiments of tumor cells demonstrated an incubation time-dependent increase in the proportion of apoptotic cells (nuclear condensation and apoptotic bodies) suggesting that autophagy and apoptosis play a role in the death of cells. Based on the obtained results, it may be considered that SB-2 is a candidate for DNA-targeting antitumor drug. Communicated by Ramaswamy H. Sarma

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