期刊
JOURNAL OF BIOMOLECULAR NMR
卷 76, 期 1-2, 页码 17-22出版社
SPRINGER
DOI: 10.1007/s10858-021-00387-5
关键词
Fucose; Glutamine synthase; Glycoprotein; Mammalian expression system; Metabolic isotope labeling; Therapeutic antibody
资金
- Taiyo Nippon Sanso Co.
- Nanotechnology Platform Program (Molecule and Material Synthesis) of MEXT
- MEXT/JSPS [JP20K15981, JP19H01017, JP19J15602]
- AMED [JP21ae0121013h0301, JP20ae0101062]
Mammalian cells are commonly used for producing recombinant glycoproteins, but the high production costs are a drawback. We developed a cost-saving method by culturing the cells under glutamine-free conditions for uniform isotope labeling.
Mammalian cells are widely used for producing recombinant glycoproteins of pharmaceutical interest. However, a major drawback of using mammalian cells is the high production costs associated with uniformly isotope-labeled glycoproteins due to the large quantity of labeled l-glutamine required for their growth. To address this problem, we developed a cost-saving method for uniform isotope labeling by cultivating the mammalian cells under glutamine-free conditions, which was achieved by co-expression of glutamine synthase. We demonstrate the utility of this approach using fucosylated and non-fucosylated Fc glycoforms of human immunoglobulin G1.
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