期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 110, 期 7, 页码 1675-1686出版社
WILEY
DOI: 10.1002/jbm.b.35028
关键词
3D printed scaffold; bioreactor; bone regeneration; critical-sized bone defect; prefabrication technique; stem cell; beta TCP; beta TCP/PCL
资金
- National Institute for Medical Research Development (NIMAD) [940547]
This study evaluated the use of the masseter muscle as a natural bioreactor for bone regeneration using beta TCP or PCL/beta TCP scaffolds. The results showed that using the masseter muscle as a bioreactor improved bone regeneration. The composition of the scaffold, pedicle preservation, and treatment with MSCs or rhBMP2 also influenced the rate of new bone formation and scaffold degradation.
In vivo bioreactors serve as regenerative niches that improve vascularization and regeneration of bone grafts. This study has evaluated the masseter muscle as a natural bioreactor for beta TCP or PCL/beta TCP scaffolds, in terms of bone regeneration. The effect of pedicle preservation, along with sole, or MSC- or rhBMP2-combined application of scaffolds, has also been studied. Twenty-four mongrel dogs were randomly placed in six groups, including beta TCP, beta TCP/rhBMP2, beta TCP/MSCs, PCL/beta TCP, PCL/beta TCP/rhBMP2, and PCL/beta TCP/MSCs. During the first surgery, the scaffolds were implanted into the masseter muscle for being prefabricated. After 2 months, each group was divided into two subgroups prior to mandibular bone defect reconstruction; one with a preserved vascularized pedicle and one without. After 12 weeks, animals were euthanized, and new bone formation was evaluated using histological analysis. Histological analysis showed that all beta-TCP scaffold groups had resulted in significantly greater rates of new bone formation, either with a pedicle surgical approach or non-pedicle surgical approach, comparing to their parallel groups of beta TCP/PCL scaffolds (p <= .05). Pedicled beta-TCP scaffold groups that were treated with either rhBMP2 (48.443% +/- 0.250%) or MSCs (46.577% +/- 0.601%) demonstrated the highest rates of new bone formation (p <= .05). Therefore, masseter muscle can be used as a local in vivo bioreactor with potential clinical advantages in reconstruction of human mandibular defects. In addition, scaffold composition, pedicle preservation, and treatment with MSCs or rhBMP2, influence new bone formation and scaffold degradation rates in the prefabrication technique.
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