期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 298, 期 1, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jbc.2021.101523
关键词
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资金
- National Science Foundation (NSF) [1804422, 1933321]
- NSF [2136421]
- Department of Ophthalmology and Visual Sciences, UTMB
- NIEHS [T32ES007254]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1804422] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [2136421] Funding Source: National Science Foundation
- Emerging Frontiers & Multidisciplinary Activities
- Directorate For Engineering [1933321] Funding Source: National Science Foundation
Oxidative stress, inflammation, and aberrant activation of microglia in the retina are commonly observed in ocular pathologies. Researchers have discovered that the binding and activation of Z-DNA-binding protein 1 (ZBP1) is triggered by the exposure of retinal pigment epithelial (RPE) cells to damaged mtDNA, leading to the expression of proinflammatory markers. Additionally, the release of extracellular vesicles containing mtDNA fragments from RPE cells induces a proinflammatory phenotype of microglia through ZBP1 signaling.
Oxidative stress, inflammation, and aberrant activation of microglia in the retina are commonly observed in ocular pathologies. In glaucoma or age-related macular degeneration, the chronic activation of microglia affects retinal ganglion cells and photoreceptors, respectively, contributing to gradual vision loss. However, the molecular mechanisms that cause activation of microglia in the retina are not fully understood. Here we show that exposure of retinal pigment epithelial (RPE) cells to (mtDNA)-specific damage, and the subsequent translocation of damaged mtDNA to the cytoplasm results in the binding and activation of intracellular DNA receptor Z-DNA-binding protein 1 (ZBP1). Activation of the mtDNA/ZBP1 pathway triggers the expression of proinflammatory markers in RPE cells. In addition, we show that the enhanced release of extracellular vesicles (EVs) containing fragments of mtDNA derived from the apical site of RPE cells induces a proinflammatory phenotype of microglia via activation of ZBP1 signaling. Collectively, our report establishes oxidatively damaged mtDNA as an important signaling molecule with ZBP1 as its intracellular receptor in the development of an inflammatory response in the retina. We propose that this novel mtDNA-mediated autocrine and paracrine mechanism for triggering and maintaining inflammation in the retina may play an important role in ocular pathologies. Therefore, the molecular mechanisms identified in this report are potentially suitable therapeutic targets to ameliorate development of ocular pathologies.
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