期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 298, 期 3, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jbc.2022.101740
关键词
-
资金
- Leibniz SAW Program
- Deutsche Forschungsgemeinschaft [HA2686/21-1]
This study reveals a mechanism for controlling lysosome biogenesis involving calcium release from the endoplasmic reticulum, independent of mechanistic target of rapamycin complex 1. The mechanism includes inositol polyphosphate-5-phosphatase A-mediated triphosphate hydrolysis, calcineurin, and the nuclear translocation and elevated transcriptional activity of TFEB/TFE3.
Lysosomes serve as dynamic regulators of cell and organismal physiology by integrating the degradation of macromolecules with receptor and nutrient signaling. Previous studies have established that activation of the transcription factor EB (TFEB) and transcription factor E3 (TFE3) induces the expression of lysosomal genes and proteins in signaling inactive starved cells, that is, under conditions when activity of the master regulator of nutrient-sensing signaling mechanistic target of rapamycin complex 1 is repressed. How lysosome biogenesis is triggered in signaling-active cells is incompletely understood. Here, we identify a role for calcium release from the lumen of the endoplasmic reticulum in the control of lysosome biogenesis that is independent of mechanistic target of rapamycin complex 1. We show using functional imaging that calcium efflux from endoplasmic reticulum stores induced by inositol triphosphate accumulation upon depletion of inositol polyphosphate-5phosphatase A, an inositol 5-phosphatase downregulated in cancer and defective in spinocerebellar ataxia, or receptor mediated phospholipase C activation leads to the induction of lysosome biogenesis. This mechanism involves calcineurin and the nuclear translocation and elevated transcriptional activity of TFEB/TFE3. Our findings reveal a crucial function for inositol polyphosphate-5-phosphatase A-mediated triphosphate hydrolysis in the control of lysosome biogenesis via TFEB/TFE3, thereby contributing to our understanding how cells are able to maintain their lysosome content under conditions of active receptor and nutrient signaling.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据