期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 297, 期 6, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jbc.2021.101434
关键词
-
资金
- NIH [GM034496, GM103622, OD018090]
- Cancer Center Support Grant [CA21765]
- American Lebanese Syrian Associated Charities
- Department of Energy Office of Science by Argonne National Laboratory [DE-AC02CH11357, DE-AC03CH11357, DE-AC04CH11357, DE-AC05CH11357, DE-AC06CH11357]
The study identified that the yiiD gene product is a malonyl-ACP decarboxylase (MadA) and showed that the expression of MadA, MadA(C), or MadB can restore normal cell size and growth rates to an E. coli ΔfabH strain.
Bacterial fatty acid synthesis in Escherichia coli is initiated by the condensation of an acetyl-CoA with a malonyl-acyl carrier protein (ACP) by the beta-ketoacyl-ACP synthase III enzyme, FabH. E. coli Delta fabH knockout strains are viable because of the yiiD gene that allows FabH-independent fatty acid synthesis initiation. However, the molecular function of the yiiD gene product is not known. Here, we show the yiiD gene product is a malonyl-ACP decarboxylase (MadA). MadA has two independently folded domains: an amino-terminal N-acetyl transferase (GNAT) domain (MadA(N)) and a carboxy-terminal hot dog dimerization domain (MadA(C)) that encodes the malonyl-ACP decarboxylase function. Members of the proteobacterial Mad protein family are either two domain MadA (GNAT-hot dog) or standalone MadB (hot dog) decarboxylases. Using structure-guided, site-directed mutagenesis of MadB from Shewanella oneidensis, we identified Asn45 on a conserved catalytic loop as critical for decarboxylase activity. We also found that MadA, MadA(C), or MadB expression all restored normal cell size and growth rates to an E. coli Delta fabH strain, whereas the expression of MadA(N) did not. Finally, we verified that GlmU, a bifunctional glucosamine-1-phosphate N-acetyl transferase/N-acetyl-glucosamine-1-phosphate uridylyltransferase that synthesizes the key intermediate UDP-GlcNAc, is an ACP binding protein. Acetyl-ACP is the preferred glucosamine-1-phosphate N-acetyl transferase/N-acetyl-glucosamine-1-phosphate uridylyltransferase substrate, in addition to being the substrate for the elongation-condensing enzymes FabB and FabF. Thus, we conclude that the Mad family of malonyl-ACP decarboxylases supplies acetyl-ACP to support the initiation of fatty acid, lipopolysaccharide, peptidoglycan, and enterobacterial common antigen biosynthesis in Proteobacteria.
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