期刊
JOURNAL OF BASIC MICROBIOLOGY
卷 62, 期 3-4, 页码 508-517出版社
WILEY
DOI: 10.1002/jobm.202100258
关键词
biodegradation; Comamonas testosteroni; phthalic acid; qPCR; viability PCR
类别
资金
- Ege University Scientific Research Projects Coordination Unit [16-Fen-023]
In this study, the degradation ability of Comamonas testosteroni strain PT9 on phthalic acid was characterized, showing complete degradation of a high concentration of phthalic acid within 6 hours. The study also investigated the stimulation of cell growth during degradation and screened for catalytic genes involved in the degradation pathways of phthalic acid isomers in strain PT9.
In this study, characterization of industry-borne Comamonas testosteroni strain PT9 isolate was performed by determining degradation ability on phthalic acid (PA). High-performance liquid chromatography analyses showed that strain PT9 completely degraded 102.94 mg/L of PA within 6 h. Viability polymerase chain reaction (vPCR) was performed with propidium monoazide treatment. vPCR showed that the PA has positively stimulated the cell growth during degradation. To consider the fate of PA, the proposed catalytic genes (ophA2, iphA2, tphA2, tphA3, pmdA, and pmdB) for the degradation pathways of PA isomers for C. testosteroni were screened in strain PT9. All genes except iphA2 were detected in strain PT9, and expression levels of related genes were analyzed by Real-Time PCR (qPCR).
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