期刊
JOURNAL OF APPLIED TOXICOLOGY
卷 42, 期 5, 页码 806-817出版社
WILEY
DOI: 10.1002/jat.4258
关键词
hepatotoxicity; inflammation; NLRP3 inflammasome; PFOS; pyroptosis; ROS
类别
资金
- Nantong Science and Technology Bureau [JC2020032, MS12018095, MS12017015-4]
- National Natural Science Foundation of China [81573188, 81902406, 81972279]
- Taizhou people's hosptal research project [ZL201916]
The study revealed that PFOS exposure activates the NLRP3 inflammasome, leading to pyroptotic death and exacerbating liver inflammation and steatosis. Inhibition of NLRP3 and pretreatment with ROS scavenger NAC can attenuate these effects.
Perfluorooctane sulfonate (PFOS) is a fluorinated organic pollutant with substantial accumulation in mammalian liver tissues. However, the impact of chronic PFOS exposure on liver disease progression and the underlying molecular mechanisms remain elusive. Herein, we for the first time revealed that micromolar range of PFOS exposure initiates the activation of NLR pyrin domain containing 3 (NLRP3) inflammasome to drive hepatocyte pyroptosis. We showed that 5 mg/kg/day PFOS exposure may exacerbated liver inflammation and steatosis in high-fat diet (HFD)-fed mice with concurrently elevated expression of NLRP3 and caspase-1. PFOS exposure resulted in viability impairment and LDH release in BRL-3A rat liver cells. 25-100 mu M concentrations of PFOS exposure activated the NLRP3 inflammasome, leading to consequent GSDMD cleavage, IL-1 beta release and the initiation of pyroptosis in a dose-dependent manner, whereas treatment with 10 mu M NLRP3 inhibitor MCC950 abrogated this effect. Moreover, pretreatment of 5 mM ROS scavenger N-acetyl-L-cysteine (NAC) ameliorated PFOS-induced NLRP3 inflammasome activation and pyroptosis. Collectively, our data highlight a pivotal role of pyroptotic death in PFOS-mediated liver inflammation and metabolic disorder.
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