Genomic analysis and characterization of Scenedesmus glucoliberatum PABB004: An unconventional sugar-secreting green alga

Aims In this report, we present Scenedesmus glucoliberatum PABB004, a microalga that was isolated from an association with Paramecium bursaria with the potential for application in fermentative processes and co-culture schemes due to its advantageous high sugar secretion phenotype. Methods and Results We sequenced, assembled and annotated the draft genome and transcriptome for this newly reported strain. The nuclear genome has an exceptionally high GC content of 78%. Our results revealed significant sugar accumulation over a range from 6.2 to 7.8 pH units. The predicted proteome was compared with other green algae that show different sugar secretion phenotypes aiming to help uncover their common features for simple sugar secretion and those unique to S. glucoliberatum PABB004. Conclusions The evolutionary history of this organism, inferred from its genomic traits, expands our current understanding of algal mutualistic relationships involving photosynthate exchanges. S. glucoliberatum PABB004 secreted ready-to-use fermentable sugars (glucose and maltose) directly to the extracellular media achieving concentrations greater than 2.7 g/L of free glucose and 1.2 g/L of maltose in batch cultures. Significance and Impact of the Study A draft genome is provided for a new member of an important class of green algae. Scenedesmus glucoliberatum PABB004 secretes high levels of simple sugars over a broad pH range.

Automated summary

This report presents a microalga, Scenedesmus glucoliberatum PABB004, with potential applications in fermentative processes and co-culture schemes due to its high sugar secretion phenotype. The genome of this strain has a high GC content of 78%. It secretes fermentable sugars at high levels over a broad pH range, expanding our understanding of algal mutualistic relationships involving sugar exchanges.