期刊
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
卷 77, 期 4, 页码 1094-1101出版社
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkab501
关键词
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资金
- UMR1184 research centre
- ANRS Maladies Infectieuses emergentes [20441]
This study investigated the distribution of antiretroviral drugs, tenofovir, emtricitabine, and dolutegravir, in mice models. The results showed that tenofovir had the highest concentration in the digestive tract, liver, and kidneys, while emtricitabine had strong penetration in most tissues. Dolutegravir had poor distribution in all tissues. These findings have important implications for future HIV therapy development.
Background Studies of antiretroviral drug (ARV) tissue distribution in preclinical models, such as mice, are key to understanding viral persistence. Objectives To determine the plasma and tissue pharmacokinetics and tissue distributions of tenofovir, emtricitabine and dolutegravir in mice. Methods ARVs were simultaneously administered to two different strains, and their levels in plasma and tissue samples were determined by a validated LC-MS/MS method. A non-compartmental analysis was performed to estimate the main pharmacokinetic parameters. A tissue penetration factor (TPF) was calculated as the ratio of the concentration in the tissue concerned to that in plasma. Results ARV plasma pharmacokinetic parameters in both strains were similar to those estimated in the clinical context. Tissue concentrations were highest in the digestive tract, followed by the liver and kidneys, lymphatic system, pancreas, adipose tissue and lungs. Tissue concentrations were lowest in the brain. Triple therapy could not be considered effective in any of the tissues considered. The TPF values obtained showed that tenofovir diffused widely, especially in the digestive tract, liver and kidneys. Emtricitabine had a TPF above 100% in two-thirds of the tissues. Dolutegravir was poorly distributed to all tissues. Conclusions Drug specificity was observed, with higher levels of exposure to tenofovir than to emtricitabine or dolutegravir. Tissue specificity was also observed, with strong penetration of the digestive tract and weak penetration of the brain. These data have important implications for future preclinical and clinical studies for developing new HIV therapies with the goal of an HIV cure.
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