期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 40, 页码 11971-11981出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c03248
关键词
Aspergillus flavus; biotin; BioDA; pathogenicity
资金
- National Natural Science Foundation of China [31772105]
The bifunctional gene bioDA in Aspergillus flavus plays a crucial role in biotin biosynthesis and can complement E. coli mutants. The gene product is mainly localized to the mitochondria, unaffected by environmental stresses, and its deletion impairs biotin production and reduces pathogenicity. This study sheds light on biotin biosynthesis in A. flavus and identifies potential antifungal drug candidates.
Biotin is an important enzyme cofactor that plays a key role in all three domains. The classical bifunctional enzyme BioDA in eukaryotes (such as Aspergillus flavus and Arabidopsis thaliana) is involved in the antepenultimate and penultimate steps of biotin biosynthesis. In this study, we identified a A. flavus bifunctional gene bioDA which could complement both Escherichia coli Delta EcbioD and Delta EcbioA mutants. Interestingly, the separated domain of AfBioD and AfBioA could, respectively, fuse with EcBioA and EcBioD well and work together. What is more, we found that BioDA was almost localized to the mitochondria in A. flavus, as shown by N-terminal red fluorescent protein tag fusion. Noteworthy, the subcellular localization of AfBioDA is never affected by common environmental stresses (such as hyperosmotic stress or oxidative stress). The knockout strategy demonstrated that the deletion of AfbioDA gene from the chromosome impaired the biotin de novo synthesis pathway in A. flavus. Importantly, this A. flavus mutant blocked biotin production and decreased its pathogenicity to infect peanuts. Based on the structural comparison, we found that two inhibitors (amiclenomycin and gemcitabine) could be candidates for antifungal drugs. Taken together, our findings identified the bifunctional AfbioDA gene and shed light on biotin biosynthesis in A. flavus.
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