期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 70, 期 5, 页码 1516-1524出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c07541
关键词
l-ergothioneine; pentose phosphate pathway; sulfur assimilation; l-cysteine pathway; l-ergothioneine synthetic pathway
资金
- National Research Foundation of Korean (NRF) - Korean government (MSIT) [2021R1A2C2006944, 2020M1A2A2080850]
- Korea University Grant
- National Research Foundation of Korea [2021R1A2C2006944, 2020M1A2A2080850] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study presents a novel strategy for synthesizing L-Ergothioneine (EGT) in Corynebacterium glutamicum, which resulted in increased EGT production. The synthesized EGT exhibited high antioxidant activity, indicating its potential for commercialization.
L-Ergothioneine (EGT) is a strong antioxidant used in industry, and it is commonly extracted from mushrooms; however, its production is limited. As an alternative, we developed metabolically engineered Corynebacterium glutamicum with reinforced sulfur assimilation and pentose phosphate pathways, which led to the accumulation of 45.0 and 63.2 mg/L EGT, respectively. Additionally, the overexpression of cysEKR resulted in further promoted EGT production in ET4 (66.5 mg/L) and ET7 (85.0 mg/L). Based on this result, we developed the strain ET11, in which all sulfur assimilatory, PP, and L-cysteine synthetic pathways were reinforced, and it synthesized 264.4 mg/L EGT. This study presents the first strategy for EGT synthesis that does not require precursor addition in C. glutamicum, and the production time was shortened. In addition, the synthesized EGT showed high radical scavenging activity (70.7%), thus confirming its antioxidant function. Consequently, this study showed the possibility of EGT commercialization by overcoming the limitations of industrial processes.
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