期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 46, 页码 13734-13743出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c05978
关键词
aphid resistance; gas chromatography-mass spectrometer; octoploid Trititrigia; RNA-seq; S-linalool synthase; volatile terpenoids
资金
- Natural Science Foundation of Shandong Province [ZR2020MC121]
- National Key R&D Program of China [2017YFD0201705]
The octoploid Trititrigia plants accumulate linalool monoterpene in response to S. avenae infestation and confer resistance to the aphids through the resistance gene OtLIS, which encodes a terpene synthase for linalool emission before and after aphid feeding.
Sitobion avenae (Fabricius) is a major insect pest of wheat worldwide that reduces crop yield and quality annually. Few germplasm resources with resistant genes to aphids have been identified and characterized. Here, octoploid Trititrigia, a species used in wheat distant hybridization breeding, was found to be repellent to S. avenae after 2 year field investigations and associated with physiological and behavioral assays. Linalool monoterpene was identified to accumulate dominantly in plants in response to S. avenae infestation. We cloned the resistance gene OtLIS by assembling the transcriptome of aphid-infested or healthy octoploid Trititrigia. Functional characterization analysis indicated that OtLIS encoded a terpene synthase and conferred resistance to S. avenae by linalool emission before and after aphid feeding. Our study suggests that the octoploid Trititrigia with the aphid-resistant gene OtLIS may have potential as a target resource for further breeding aphid-resistant wheat cultivars.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据