期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 70, 期 4, 页码 976-991出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c06750
关键词
food safety; IgY; immunoassay; contaminants
资金
- National Science Foundation of China [31873025]
- Chinese Ministry of Science and Technology (MOST) [2018YFC1602900]
This study discussed the application of chicken yolk antibody (IgY) in detecting chemical and biological hazards in food samples, presenting its advantages, structure, production methods, and principles of immunoassays. The future requires more types of IgY, increased sensitivity, and diversification in recognition-to-signal methods.
As antibodies are the main biological binder for hazards in food samples, their performance directly determines the sensitivity, specificity, and reproducibility of the developed immunoassay. The overwhelmingly used mammalian-derived antibodies usually suffer from complicated preparation, high cost, frequent bleeding of animals, and sometimes low titer and affinity. Chicken yolk antibody (IgY) has recently attracted considerable attention in the bioanalytical field owing to its advantages in productivity, animal welfare, comparable affinity, and high specificity. However, a broad understanding of the application of IgY-based immunoassay for the detection of chemical and biological hazards in food samples remains limited. Here, we briefly summarized the diversity, structure, and production of IgY including polyclonal and monoclonal formats. Then, a comprehensive overview of the principles, designs, and applications of IgY-based immunoassays for these hazards was reviewed and discussed, including food-borne pathogens, food allergens, veterinary drugs, pesticides, toxins, endocrine disrupting chemicals, etc. Thus, the trend of IgY-based immunoassays is expected, and more IgY types, higher sensitivity, and diversification of recognition-to-signal manners are necessary in the future.
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