期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 70, 期 4, 页码 1058-1067出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c07046
关键词
salivary protein; Sitobion miscanthi; Buchnera aphidicola; GroES; defense response
资金
- National Natural Science Foundation of China [32001900]
- China Postdoctoral Science Foundation [2020M680786]
- National Key RD Plan of China [2017YFD0201700]
- Earmarked fund for China Agriculture Research System [CARS-22]
- MARA-CABI Joint Laboratory for Bio-safety
Salivary proteins secreted by aphids, such as GroES, play a crucial role in regulating plant defense responses. The presence of GroES in wheat aphid saliva induces hydrogen peroxide accumulation, callose deposition, and activates the plant's defense pathways. This suggests that plants have evolved mechanisms to detect aphid attack through recognizing proteins derived from Buchnera aphidicola.
Salivary proteins secreted by aphids during feeding play an important role in regulating the plant defense response. We used mass spectrometry to identify 155 proteins from the wheat aphid, Sitobion miscanthi, among which 44 proteins were derived from the primary symbiont, Buchnera aphidicola. GroES, which is a highly abundant molecular chaperone that binds to GroEL, was detected in saliva. In vitro injection of purified GroES protein and overexpression of GroES in wheat leaves verified that GroES induced hydrogen peroxide accumulation and callose deposition in wheat and further activated the plant salic acid and jasmonic acid defense pathways. Our findings indicate that plants may have evolved new strategies to detect aphid attack and trigger defense responses by recognizing proteins derived from B. aphidicola, which is present in almost all aphid species.
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