期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 41, 页码 12344-12353出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c04197
关键词
ambient ionization; volatile analysis; high-throughput; DART; smoke taint; Brettanomyces
资金
- NIFA Predoctoral Fellowships Program [2021-67034-34971]
- Agriculture and Food Research Initiative from the United States Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) [2017-67007-25940]
- NY Wine Grape Foundation Enology Program [I-34]
- National Science Foundation [ECCS-15420819]
- NIFA [914502, 2017-67007-25940] Funding Source: Federal RePORTER
The study describes modifications to a previous SPMESH extraction device, enabling parallel extraction of low-volatility analytes from multiwell plates under direct immersion conditions for rapid quantitation. The DI-SPMESH-DART-MS approach showed good accuracy in real grape juice samples, making it potentially useful for prescreening a large number of samples.
Poly(dimethylsiloxane)-based thin-film sorbent sheets (SPMESH) have previously been used for parallel headspace (HS) extraction prior to direct analysis in real-time mass spectrometry (DART-MS) for rapid quantitation of odorants in complex matrices. However, HS-SPMESH extraction is poorly suited for less volatile odorants, e.g., volatile phenols. This report describes modifications to the previous SPMESH extraction device, which make it amenable to parallel extraction of low-volatility analytes from multiwell plates under direct immersion (DI) conditions. Optimization and validation of the DI-SPMESH-DART-MS approach were performed on four volatile phenols (4-ethylphenol, 4-ethylguaiacol, 4-methylguaiacol, and guaiacol) of relevance to the quality of grape juices. Negative-ion mode DART-MS spectra showed a series of oxygenated adducts [M + nO - H](-) for all analytes, but isobaric interferences could be limited for three of the four analytes by selecting an appropriate MS/MS transition. Signal suppression from nonvolatiles (sugars, acids) could be overcome by a rinse step. DI-SPMESH-DART-MS analysis of 24 samples could be performed in similar to 45 min (30 min extraction, 16 min DART analysis) with 0.5-3 mu g/L detection limits in aqueous and model juice solutions. In real grape juices (n = 5 cultivars), good accuracy (72-137%) could be achieved for two of the four volatile phenols initially investigated, 4-ethylphenol and 4-ethylguaiacol. However, poor accuracy was observed for guaiacol in some cultivars, and 4-methylguaiacol could not be quantitated due to interferences with other volatile phenols. Despite these limitations, DI-SPMESH-DART-MS/MS may be useful for prescreening a large number of samples prior to more selective conventional analyses.
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