期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 70, 期 2, 页码 543-553出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.1c06513
关键词
flavedo; polymethoxylated flavonoid; O-methylation; microarray analysis; citrus
资金
- KAKENHI from Japan Society for the Promotion of Science (JSPS) [JP20H02976, JP19K06030]
Citrus fruits with high levels of nobiletin accumulation showed high expression of CitOMT2, while fruits with low levels of nobiletin had low expression of CitOMT2. Functional analysis revealed that CitOMT2 acts as a caffeic acid 8-O-methyltransferase, playing a crucial role in nobiletin biosynthesis.
Nobiletin (3',4',5,6,7,8-hexamethoxyflavone) is a polymethoxylated flavonoid specifically accumulated in citrus fruit with numerous beneficial effects to human health. In this study, a novel O-methyltransferase (CitOMT2) was isolated from three citrus varieties, Ponkan mandarin (Citrus reticulata Blanco), Nou 6 (King mandarin x Mukaku-kishu), and Satsuma mandarin (Citrus unshiu Marc.), and its functions were characterized in vitro. The gene expression results showed that CitOMT2 was highly expressed in the two nobiletin abundant varieties of Ponkan mandarin and Nou 6. However, the expression level of CitOMT2 was low in the flavedo of Satsuma mandarin, in which only a small amount of nobiletin was accumulated. Functional analysis suggested that CitOMT2 was a caffeic acid 8-O-methyltransferase, and it catalyzed the O-methylation of 7,8-dihydroxyflavone at 8-OH. As the methylation of flavone at 8-OH was required for nobiletin biosynthesis, the results presented in this study suggested that CitOMT2 was a key gene regulating nobiletin accumulation in citrus fruit.
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