期刊
IONICS
卷 27, 期 12, 页码 5323-5331出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11581-021-04252-1
关键词
Electrochemical immunosensor; Polydimer(p-aminobenzoic acid); Okadaic acid; Modified electrode; Gold three-dimensional nanoelectrode ensembles
资金
- COMRA project [DY135-B2-17]
- National Natural Science Foundation of China [21273056]
An ultrasensitive label-free electrochemical immunosensor for detecting okadaic acid (OA) was developed using p-aminobenzoic acid-modified gold three-dimensional nanoelectrode ensembles (3DNEEs). The sensor showed a high detection accuracy and a wide linear range for OA in oyster samples, demonstrating its potential application value in food safety and environmental monitoring.
An ultrasensitive label-free electrochemical immunosensor of okadaic acid (OA) was studied based on electrodimerization of p-aminobenzoic acid (p-ABA)-modified gold three-dimensional nanoelectrode ensembles (3DNEEs) which were prepared by electroless gold plating and etching using polycarbonate as template. Staphylococcus protein A (SPA) was introduced to immobilize okadaic acid antibody (anti-OA). The morphology and composition of 3DNEEs were analyzed by scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometer (EDX). The polydimer (p-aminobenzoic acid) (pPABA) film was measured by Fourier transform infrared spectrometer (FTIR). The concentration of OA was measured by EIS and SWV. The detection limit of EIS is lower than that of SWV (6.47 x 10(-3) ng mL(-1) vs. 9.15 x 10(-3) ng mL(-1)), but the linear OA detection range of the latter is wider (0.01-1.0 ng mL(-1) vs. 0.01-0.8 ng mL(-1)). The average recovery of OA in oyster samples is 99.81%. The OA recovery test in oyster samples demonstrated potential application value of the electrochemical immunosensor.
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