Nocardioides humilatus sp. nov., isolated from farmland soil in the Republic of Korea

A Gram-stain positive, aerobic, irregularly rod-shaped, non-spore-forming bacterium, designated as BN130099(T), was isolated from farmland soil sampled in Goesan-gun, Chungbuk, Republic of Korea. Phylogenetic analysis of its 16S rRNA gene sequence showed that the strain is closely related to Nocardioides pelophilus KACC 19192(T) with 98.11 % similarity. The DNA G+C content of strain BN130099(T) was 68.84 mol% (draft genome sequence). The genome sequence of BN130099(T) displayed key enzymes involved in bioremediation of organic pollutants and biosynthetic clusters of saquayamycin. The strain contained LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H-4) as the major respiratory quinone. The predominant fatty acid was iso-C-16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The results of physiological and biochemical characterization allowed the phenotypic differentiation of strain BN130099(T) from N. pelophilus KACC 19192(T). The strain represents a novel species of the genus Nocardioides, for which we propose the name Nocardioides humilatus sp. nov. The type strain is BN130099(T) (=KCTC 49079(T)=CCTCC AB 2018135(T)).

Automated summary

A new bacterium species, Nocardioides humilatus, was isolated from farmland soil in Goesan-gun, Chungbuk, Republic of Korea. It showed potential for bioremediation and biosynthesis.