期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 17, 期 -, 页码 783-797出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S345357
关键词
bioceramics; scaffolds; surface modification; element doping; bone regeneration
资金
- National Natural Science Foundation of China [81771115]
- Natural Science Foundation of Shanghai [20ZR1431000]
- Science and Technology Commission of Shanghai Municipality [19441906200]
- Shanghai Rising-Star Program [19QA1405200]
- CSA Clinical Research Fund [CSA-O2020-04]
- Innovative Research Team of High-level Local Universities in Shanghai [SSMU-ZDCX20180902]
This study investigates the synergistic effects of Sr doping and surface modification on the osteogenic and angiogenic activities of HAp bioceramics. It was found that HAp bioceramics with micro-nano-hybrid surfaces and Sr doping could significantly enhance cell proliferation, ALP activity, and the expression of osteogenic and angiogenic factors. In vivo experiments also revealed that Sr-doped HAp bioceramics promoted bone and blood vessel regeneration in rat calvarial defects.
Background: The synergistic effect of chemical element doping and surface modification is considered a novel way to regulate cell biological responses and improve the osteoinductive ability of biomaterials. Methods: Hydroxyapatite (HAp) bioceramics with micro-nano-hybrid (a mixture of microrods and nanorods) surfaces and different strontium (Sr) doping contents of 2.5, 5, 10, and 20% (Srx-mnHAp, x: 2.5, 5, 10 and 20%) were prepared via a hydrothermal transformation method. The effect of Srx-mnHAp on osteogenesis and angiogenesis of bone marrow stromal cells (BMSCs) was evaluated in vitro, and the bioceramics scaffolds were further implanted into rat calvarial defects for the observation of bone regeneration in vivo. Results: HAp bioceramics with micro-nano-hybrid surfaces (mnHAp) could facilitate cell spreading, proliferation ability, ALP activity, and gene expression of osteogenic and angiogenic factors, including COL1, BSP, BMP-2, OPN, VEGF, and ANG-1. More importantly, Srx-mnHAp (x: 2.5, 5, 10 and 20%) further promoted cellular osteogenic activity, and Sr10-mnHAp possessed the best stimulatory effect. The results of calvarial defects revealed that Sr10-mnHAp could promote more bone and blood vessel regeneration, with mnHAp and HAp bioceramics (dense and flat surfaces) as compared. Conclusion: The present study suggests that HAp bioceramics with micro-nano-hybrid surface and Sr doping had synergistic promotion effects on bone regeneration, which can be a promising material for bone defect repair.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据