期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 21, 页码 -出版社
MDPI
DOI: 10.3390/ijms222111536
关键词
Mycobacterium smegmatis; small non-coding RNA; F6; resuscitation promoting factor RpfE2; dormancy; non-culturability; adaptation to stresses
资金
- RFBR [16-04-01247]
- Russian Federation
Regulatory small non-coding RNA F6 in M. smegmatis plays an essential role in bacterial adaptation to environmental stresses, contributing to resistance against oxidative stress and influencing the expression of RpfE2, a resuscitation-promoting factor. The deletion of F6 results in reduced ability for dormancy entry in potassium deficiency conditions, highlighting the significance of F6 in bacterial adaptation to suboptimal growth conditions.
Regulatory small non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. Various stresses such as hypoxia and nutrient starvation cause a reduction in the metabolic activity of Mycobacterium smegmatis, leading to entry into dormancy. We investigated the functional role of F6, a small RNA of M. smegmatis, and constructed an F6 deletion strain of M. smegmatis. Using the RNA-seq approach, we demonstrated that gene expression changes that accompany F6 deletion contributed to bacterial resistance against oxidative stress. We also found that F6 directly interacted with 5 & PRIME;-UTR of MSMEG_4640 mRNA encoding RpfE2, a resuscitation-promoting factor, which led to the downregulation of RpfE2 expression. The F6 deletion strain was characterized by the reduced ability to enter into dormancy (non-culturability) in the potassium deficiency model compared to the wild-type strain, indicating that F6 significantly contributes to bacterial adaptation to non-optimal growth conditions.
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