期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/ijms23020684
关键词
plant respiration; plant mitochondria; RNA editing; structure-function
资金
- U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-SC0022293]
- U.S. Department of Energy (DOE) [DE-SC0022293] Funding Source: U.S. Department of Energy (DOE)
This study uses the structures of plant respiratory complexes to analyze the impact of RNA editing on amino acid changes, providing a molecular explanation for the phenotypes of RNA editing mutants. The research bridges the gap between sequence and phenotype and offers a framework for further analysis of RNA editing mutants in plant mitochondria and chloroplasts.
The last steps of respiration, a core energy-harvesting process, are carried out by a chain of multi-subunit complexes in the inner mitochondrial membrane. Several essential subunits of the respiratory complexes are RNA-edited in plants, frequently leading to changes in the encoded amino acids. While the impact of RNA editing is clear at the sequence and phenotypic levels, the underlying biochemical explanations for these effects have remained obscure. Here, we used the structures of plant respiratory complex I, complex III2 and complex IV to analyze the impact of the amino acid changes of RNA editing in terms of their location and biochemical features. Through specific examples, we demonstrate how the structural information can explain the phenotypes of RNA-editing mutants. This work shows how the structural perspective can bridge the gap between sequence and phenotype and provides a framework for the continued analysis of RNA-editing mutants in plant mitochondria and, by extension, in chloroplasts.
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