期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 21, 页码 -出版社
MDPI
DOI: 10.3390/ijms222111750
关键词
pannexin1b; zebrafish; retinal ganglion cells; Muller glia; circadian clock; visual system; transcriptomics; behavior
资金
- Natural Sciences and Engineering Research Council (NSERC) [RGPIN-418142-2012]
Panx1a and Panx1b are genes in zebrafish that play roles in the physiology of the visual system, with unique channel properties and tissue expression patterns. Panx1a modulates light decrement detection in the outer retina, while Panx1b's strategic localization in the inner retina affects visually evoked motor behavior. Loss of Panx1b channels disrupts retinal responses to changes in illumination and impairs larval locomotion in low light conditions, suggesting its involvement in motion detection and circadian clock regulation.
Pannexin1 (Panx1) can form ATP-permeable channels that play roles in the physiology of the visual system. In the zebrafish two ohnologs of Panx1, Panx1a and Panx1b, have unique and shared channel properties and tissue expression patterns. Panx1a channels are located in horizontal cells of the outer retina and modulate light decrement detection through an ATP/pH-dependent mechanisms and adenosine/dopamine signaling. Here, we decipher how the strategic localization of Panx1b channels in the inner retina and ganglion cell layer modulates visually evoked motor behavior. We describe a panx1b knockout model generated by TALEN technology. The RNA-seq analysis of 6 days post-fertilization larvae is confirmed by real-time PCR and paired with testing of locomotion behaviors by visual motor and optomotor response tests. We show that the loss of Panx1b channels disrupts the retinal response to an abrupt loss of illumination and it decreases the larval ability to follow leftward direction of locomotion in low light conditions. We concluded that the loss of Panx1b channels compromises the final output of luminance as well as motion detection. The Panx1b protein also emerges as a modulator of the circadian clock system. The disruption of the circadian clock system in mutants suggests that Panx1b could participate in non-image forming processes in the inner retina.
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