期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 24, 页码 -出版社
MDPI
DOI: 10.3390/ijms222413535
关键词
potato; genome-wide; CIPK gene family; gene expression; drought and osmotic tolerance; ABA
资金
- State Key Laboratory of Aridland Crop Science, Gansu Agricultural University [GSCS-2019-Z03]
- National Natural Science Foundation of China [31860399, 31960444]
The study revealed that CIPK genes in potatoes play significant roles in plant growth, development, and stress tolerance, with StCIPK10 being particularly important in enhancing potato's tolerance to drought and osmotic stress. StCIPK10 acts at the intersection of abscisic acid and stress signaling pathways, controlling root growth and stomatal closure in potatoes, and interacts with various CBL proteins to modulate plant responses to environmental stresses.
The potato (Solanum tuberosum L.), one of the most important food crops worldwide, is sensitive to environmental stresses. Sensor-responder complexes comprising calcineurin B-like (CBL) proteins and CBL-interacting protein kinases (CIPKs) not only modulate plant growth and development but also mediate numerous stress responses. Here, using a Hidden Markov Model and BLAST searches, 27 CIPK genes were identified in potato and divided into five groups by phylogenetic analysis and into two clades (intron-poor and intron-rich) by gene structure analysis. Quantitative reverse-transcription PCR (qRT-PCR) assays revealed that StCIPK genes play important roles in plant growth, development and abiotic stress tolerance. Up-regulated expression of StCIPK10 was significantly induced by drought, PEG6000 and ABA. StCIPK10 enhances both the ability of potato to scavenge reactive oxygen species and the content of corresponding osmoregulation substances, thereby strengthening tolerance to drought and osmotic stress. StCIPK10 is located at the intersection between the abscisic acid and abiotic stress signaling pathways, which control both root growth and stomatal closure in potato. In addition, StCIPK10 interacts with StCBL1, StCBL4, StCBL6, StCBL7, StCBL8, StCBL11 and StCBL12, and is specifically recruited to the plasma membrane by StCBL11.
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