期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/ijms23031409
关键词
protein disulfide isomerase; AtPDI11; D domain; oxidative protein folding
资金
- China Postdoctoral Science Foundation [2019M663795]
- Natural Science Basic Research Program of Shaanxi [2020JQ-970]
- Agricultural Technology Research and Development Project of Xi'an Science and Technology Bureau [20NYYF0058]
- Key Research and Development Plan Project of Shaanxi Province [2018ZDXM-SF-016]
- Key Laboratory Research Fund of Department of Education of Shaanxi Province [18JS111]
This study reveals the evolutionary conservation of PDI-S between land plants and algal organisms, and elucidates the induction mechanism of AtPDI11 under ER stress and the crucial importance of the D domain for its activity.
The formation and isomerization of disulfide bonds mediated by protein disulfide isomerase (PDI) in the endoplasmic reticulum (ER) is of fundamental importance in eukaryotes. Canonical PDI structure comprises four domains with the order of a-b-b '-a '. In Arabidopsis thaliana, the PDI-S subgroup contains only one member, AtPDI11, with an a-a '-D organization, which has no orthologs in mammals or yeast. However, the expression pattern of AtPDI11 and the functioning mechanism of AtPDI11 D domain are currently unclear. In this work, we found that PDI-S is evolutionarily conserved between land plants and algal organisms. AtPDI11 is expressed in various tissues and its induction by ER stress is disrupted in bzip28/60 and ire1a/b mutants that are null mutants of key components in the unfolded protein response (UPR) signal transduction pathway, suggesting that the induction of AtPDI11 by ER stress is mediated by the UPR signaling pathway. Furthermore, enzymatic activity assays and genetic evidence showed that the D domain is crucially important for the activities of AtPDI11. Overall, this work will help to further understand the working mechanism of AtPDI11 in catalyzing disulfide formation in plants.
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