期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/ijms23031396
关键词
Desulfovibrio alaskensis G20; sulfate reducing bacteria; RNA sequencing; transcriptomics; gene expression; copper; toxicity; oxidative stress; homeostasis; gene ontology
资金
- National Science Foundation [1736255, 1849206, 1920954]
- Department of Chemical and Biological Engineering at the South Dakota School of Mines and Technology
- Office Of The Director
- Office of Integrative Activities [1920954] Funding Source: National Science Foundation
This study reported the effects of Cu(II) stress on a model SRB, Desulfovibrio alaskensis G20. The results indicated that the expression of genes was massively modulated at different Cu(II) concentrations, and changes in inorganic ion transporters, translation machinery, DNA transcription, and signal transduction were observed.
Copper (Cu) is an essential micronutrient required as a co-factor in the catalytic center of many enzymes. However, excess Cu can generate pleiotropic effects in the microbial cell. In addition, leaching of Cu from pipelines results in elevated Cu concentration in the environment, which is of public health concern. Sulfate-reducing bacteria (SRB) have been demonstrated to grow in toxic levels of Cu. However, reports on Cu toxicity towards SRB have primarily focused on the degree of toxicity and subsequent elimination. Here, Cu(II) stress-related effects on a model SRB, Desulfovibrio alaskensis G20, is reported. Cu(II) stress effects were assessed as alterations in the transcriptome through RNA-Seq at varying Cu(II) concentrations (5 mu M and 15 mu M). In the pairwise comparison of control vs. 5 mu M Cu(II), 61.43% of genes were downregulated, and 38.57% were upregulated. In control vs. 15 mu M Cu(II), 49.51% of genes were downregulated, and 50.5% were upregulated. The results indicated that the expression of inorganic ion transporters and translation machinery was massively modulated. Moreover, changes in the expression of critical biological processes such as DNA transcription and signal transduction were observed at high Cu(II) concentrations. These results will help us better understand the Cu(II) stress-response mechanism and provide avenues for future research.
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