期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 19, 页码 -出版社
MDPI
DOI: 10.3390/ijms221910399
关键词
G-quadruplex; c-MYC; 9-methoxyluminarine; fluorescence; CD spectroscopy
The highly fluorescent 9-methoxyluminarine ligand 9-MeLM was found to interact with different topologies of G-quadruplexes, selectively quenched by parallel c-MYC G-quadruplex DNA. This suggests that 9-MeLM can potentially be used as a selective probe for parallel G-quadruplexes.
The interactions of G-quadruplexes of different topologies with highly fluorescent 9-methoxyluminarine ligand 9-MeLM were investigated by fluorescence and circular dichroism spectroscopy. The results showed that 9-methoxyluminarine was able to interact and did not destabilize any investigated molecular targets. The studied compound was selectively quenched by parallel c-MYC G-quadruplex DNA, whereas hybrid and antiparallel G4 topology caused only a negligible decrease in the fluorescence of the ligand. A high decrease of fluorescence of the ligand after binding with c-MYC G-quadruplex suggests that this molecule can be used as a selective probe for parallel G-quadruplexes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据