期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 24, 页码 -出版社
MDPI
DOI: 10.3390/ijms222413203
关键词
Flightless; squamous cell carcinoma; cancer metastasis; non-melanoma skin cancer
资金
- Australian Postgraduate Award scholarship
- Cell Therapy Manufacturing Cooperative Research Centre funding
- Channel 7 Children's Research Foundation Fellowship
- NHMRC Senior Research Fellowship GNT [1102617]
- National Health and Medical Research Council of Australia [1102617] Funding Source: NHMRC
Cutaneous squamous cell carcinoma (cSCC) is a common type of skin cancer in Caucasian populations, with aggressive metastatic forms having poor patient outcomes. Studies have shown that the actin-remodeling protein Flightless (Flii) plays a role in regulating cSCC proliferation and tumor progression through the Wnt/β-catenin signaling pathway. Knockdown of Flii led to decreased β-catenin and a reduction in the downstream effector protein SOX9, indicating its potential as a target for aggressive metastatic cSCC treatment.
Cutaneous squamous cell carcinoma (cSCC) accounts for 25% of cutaneous malignancies diagnosed in Caucasian populations. Surgical removal in combination with radiation and chemotherapy are effective treatments for cSCC. Nevertheless, the aggressive metastatic forms of cSCC still have a relatively poor patient outcome. Studies have linked actin cytoskeletal dynamics and the Wnt/beta-catenin signaling pathway as important modulators of cSCC pathogenesis. Previous studies have also shown that the actin-remodeling protein Flightless (Flii) is a negative regulator of cSCC. The aim of this study was to investigate if the functional effects of Flii on cSCC involve the Wnt/beta-catenin signaling pathway. Flii knockdown was performed using siRNA in a human late stage aggressive metastatic cSCC cell line (MET-1) alongside analysis of Flii genetic murine models of 3-methylcholanthrene induced cSCC. Flii was increased in a MET-1 cSCC cell line and reducing Flii expression led to fewer PCNA positive cells and a concomitant reduction in cellular proliferation and symmetrical division. Knockdown of Flii led to decreased beta-catenin and a decrease in the expression of the downstream effector of beta-catenin signaling protein SOX9. 3-Methylcholanthrene (MCA)-induced cSCC in Flii overexpressing mice showed increased markers of cancer metastasis including talin and keratin-14 and a significant increase in SOX9 alongside a reduction in Flii associated protein (Flap-1). Taken together, this study demonstrates a role for Flii in regulating proteins involved in cSCC proliferation and tumor progression and suggests a potential role for Flii in aggressive metastatic cSCC.
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