期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/ijms222212544
关键词
lysin; Staphylococcus aureus; silica-binding peptide; antimicrobial agents immobilization; surface functionalization; antimicrobial agents; biofilm
资金
- National Natural Science Foundation of China [32070187, 31770192]
- Youth Innovation Promotion Association CAS
- CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology [2021SPCAS001]
The study involves incorporating silica-binding peptide and anti-staphylococcal lysin into functional coatings to kill Staphylococcus aureus and prevent biofilm formation. SiBP1-ClyF-functionalized coatings effectively kill MRSA strains and support normal growth of mammalian cells on surfaces.
Staphylococcal-associated device-related infections (DRIs) represent a significant clinical challenge causing major medical and economic sequelae. Bacterial colonization, proliferation, and biofilm formation after adherence to surfaces of the indwelling device are probably the primary cause of DRIs. To address this issue, we incorporated constructs of silica-binding peptide (SiBP) with ClyF, an anti-staphylococcal lysin, into functionalized coatings to impart bactericidal activity against planktonic and sessile Staphylococcus aureus. An optimized construct, SiBP1-ClyF, exhibited improved thermostability and staphylolytic activity compared to its parental lysin ClyF. SiBP1-ClyF-functionalized coatings were efficient in killing MRSA strain N315 (> 99.999% within 1 h) and preventing the growth of static and dynamic S. aureus biofilms on various surfaces, including siliconized glass, silicone-coated latex catheter, and silicone catheter. Additionally, SiBP1-ClyF-immobilized surfaces supported normal attachment and growth of mammalian cells. Although the recycling potential and long-term stability of lysin-immobilized surfaces are still affected by the fragility of biological protein molecules, the present study provides a generic strategy for efficient delivery of bactericidal lysin to solid surfaces, which serves as a new approach to prevent the growth of antibiotic-resistant microorganisms on surfaces in hospital settings and could be adapted for other target pathogens as well.
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