期刊
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
卷 19, 期 2, 页码 353-363出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijms.68971
关键词
skeletal muscle satellite cells; mesenchymal stem cells; binge ethanol; inflammation; liver; gut
资金
- Basic Science Research Program through the National Research Foundation of Korea [2017R1D1A1A02019212, 2017R1D1A1A02018088, 2017H1A2A1045727]
- National Research Foundation of Korea [2017R1D1A1A02019212, 2017H1A2A1045727, 2017R1D1A1A02018088] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Cultured human skeletal-muscle satellite cells (SkMSCs) have shown anti-inflammatory properties by secreting prostaglandin E2 and hepatocyte growth factor (HGF). In this study, it was found that a single treatment with SkMSCs could ameliorate acute liver and gut inflammation induced by binge ethanol administration in mice, reducing markers of liver injury, bacterial-derived lipopolysaccharide levels, tissue inflammation, and leaky gut indicators.
Cultured human skeletal-muscle satellite cells have properties of mesenchymal stem cells (skeletal muscle satellite cell-derived mesenchymal stem cells, SkMSCs) and play anti-inflammatory roles by secreting prostaglandin E2 and hepatocyte growth factor (HGF). To evaluate the utility of SkMSCs in treating liver diseases, we determined whether SkMSCs could ameliorate acute liver and gut inflammation induced by binge ethanol administration. Binge drinking of ethanol led to weight loss in the body and spleen, liver inflammation and steatosis, and increased serum ALT and AST levels (markers of liver injury), along with increased IL-1 beta, TNF-alpha, and iNOS expression levels in mice. However, levels of these binge-drinking-induced indicators were reduced by a single intraperitoneal treatment of SkMSCs. Furthermore, levels of bacteria-derived lipopolysaccharide decreased in the livers and sera of ethanol-exposed mice after SkMSC administration. SkMSCs decreased the extent of tissue inflammation and reduced villus and crypt lengths in the small intestine after alcohol binge drinking. SkMSCs also reduced the leakage of blood albumin, an indicator of leaky gut, in the stool of ethanol-exposed mice. Alcohol-induced damage to human colonic Caco-2/tc7 cells was also alleviated by HGF. Therefore, a single treatment with SkMSCs can attenuate alcoholic liver damage by reducing inflammatory responses in the liver and gut, suggesting that SkMSCs could be used in cell therapy to treat alcoholic liver diseases.
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