ELISpot assay and prediction of organ transplant rejection

The ELISpot assay is a sensitive technique applied to assess cytokine-producing memory/effector T cells and human leukocyte antigens (HLA)-specific IgG-producing B cells. Besides the fact that the method is laborious and is difficult to standardise between laboratories, it may provide valuable information on the immune response of recipients before and after organ transplantation. In this article, we briefly review the recent literature and discuss the clinical significance of the ELISpot assay in predicting the risk and incidence of allograft rejection and survival.

Automated summary

The ELISpot assay is a sensitive technique used to assess immune responses, and despite being difficult to standardize, it can provide valuable information for organ transplant recipients.