Voltage generation by photosystem I complexes immobilized onto a millipore filter under continuous illumination

Here we measured the long-term photovoltage (Alf) generation by photosynthetic pigment-protein complex of photosystem I (PS I) from cyanobacterium Synechocystis sp. PCC 6803 directly. Prior to measurements PS I was immobilized onto a Millipore membrane filter (MF) and sandwiched between two semiconductor indium tin oxide (ITO) electrodes. The ability of ITO|PS I-MF|ITO system to generate a stable delta V & nbsp;over a 50 min period of irradiation by white light was detected in the presence of cytochrome c(6), methyl viologen and disaccharide trehalose. Re-injection of a fresh buffer into ITO|PS I-MF|ITO preserves the immobilized PS I functionality after six months of storage at 23 & DEG;C in the dark. The developed system represents a simple bio-hybrid solar converter to obtain voltage signals under long-term, periodic illumination. This approach can also be used to couple photo active (light-driven enzymes) and catalytic modules to generate H-2 under the steady-state conditions.& nbsp;(c) 2022 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

Automated summary

This study directly measured the long-term photovoltage generation by the photosynthetic pigment-protein complex of photosystem I (PS I) from cyanobacterium Synechocystis sp. PCC 6803. By immobilizing PS I onto a membrane filter and sandwiching it between two semiconductor electrodes, a stable photovoltage system was successfully developed. The results demonstrated that the system could generate a stable voltage signal under certain conditions.