Genome-wide identification and functional analysis of the glutathione S-transferase (GST) family in Pomacea canaliculata

Cold causes oxidative stress in living organisms, mainly caused by the accumulation of reactive oxygen species (ROS). In the antioxidant defense systems, Glutathione S-transferases (GSTs) play a vital role in the regulation of detoxification and redox balance of ROS. In this study, the P. canaliculata GST gene family were characterized using a genome-wide search of the conserved domain. Phylogenetic tree and domain composition analysis revealed that 30 PcGSTs belong to seven classes, including five in MAPEG, two in Mu, nine in Omega, 11 in Sigma, one in Rho, Theta and Alpha class, respectively. RNA-seq analysis revealed that most PcGSTs localized in mitochondria highly expressed in hepatopancreas, and most PcGSTs localized in cytoplasm highly expressed in kidney. A total of 12 PcGST genes were significantly up-regulated and PcGST12 was significantly down-regulated after cold acclimation. Ten PcGSTs were identified as DEGs under cold stress after cold acclimation. qRT-PCR revealed that the expression level of five PcGST genes were significantly varied during the cold acclimation. The present study investigated the characterization of the P. canaliculata GST gene family, extending our understanding of GST mediated cold acclimation and cold stress-response mechanisms in this invasive snail.

Automated summary

This study investigated the role of Glutathione S-transferases (GSTs) in cold acclimation and cold stress-response mechanisms in the invasive snail P. canaliculata. A total of 30 PcGSTs were classified into seven classes and their expression patterns in different tissues were examined. RNA-seq analysis and qRT-PCR experiments revealed significant changes in expression levels of certain PcGST genes during cold acclimation, providing insights into the mechanisms underlying cold stress responses.