期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 188, 期 -, 页码 983-992出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.08.077
关键词
Laccase; Lignin polymerization; Secondary wall development
资金
- National Natural Science Foundation of China [31971618]
- 111 Project [B13007]
PtrLAC16 is a crucial Populus laccase that directly affects the polymerization of lignin monomers, especially sinapyl alcohol lignin, and plays a significant role in the formation of xylem cell walls in Populus.
Plant laccases have been proposed to participate in lignin biosynthesis. However, there is no direct evidence that individual laccases in Populus can polymerize lignin monomers and alter cell wall structure. Here, a Populus laccase, PtrLAC16, was expressed and purified in a eukaryotic system. Enzymatic analysis of PtrLAC16 showed that it could polymerize lignin monomers in vitro. PtrLAC16 preferred sinapyl alcohol, and this preference is associated with an altered S/G ratio in transgenic Populus lines. PtrLAC16 was localized exclusively in the cell walls of stem vascular tissue, and a reduction in PtrLAC16 expression led to a significant decrease in lignin content and altered cell wall structure. There was a direct correlation between the inhibition of PtrLAC16 expression and structural changes in the stem cell wall of Populus. This study provides direct evidence that PtrLAC16 plays a key role in the polymerization of lignin monomers, especially for sinapyl lignin, and affects the formation of xylem cell walls in Populus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据