期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 191, 期 -, 页码 192-200出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.09.081
关键词
Protein corona; Macrophages; Au nanoparticles; NP-cell interactions
The study demonstrated that time-related protein corona on nanoparticles can influence macrophage polarization, inducing M2 phenotype at early stages and M1 phenotype at later stages. Proteomic analysis showed constant changes in protein corona compositions with significant increase of immune proteins after 12 hours of incubation, playing a key role in modulating macrophage polarization.
When nanoparticles (NPs) come into contact with bioenvironments, a protein corona forms on the NP surface. Previous reports showed that the constituents of the corona change with time. However, how different protein corona compositions influence cells, especially immune cells, has received less attention. Macrophages are important immune cells that can be polarized into a pro-inflammatory (M1) or anti-inflammatory (M2) phenotype. In this study, AuNPs were incubated with human plasma for different periods to obtain time-related AuNP-coronas, and the influences of time-related AuNP-coronas on macrophage polarization were investigated. The macrophage morphology, biomarkers, cytokine secretion studies show that the pristine AuNPs and 4 hAuNP-corona induced macrophage cells into M2 phenotype, while the co-incubation of 12 h-AuNP-corona and macrophage cells result in M1 phenotype. Further proteomic analysis showed that the compositions of protein corona were changing constantly after AuNPs contacted with plasma. When the incubation time increased to 12 h, the immune proteins in protein corona were increased significantly, which play a key role in modulation of the different macrophages polarization. Our findings demonstrated that plasma incubation time is an important parameter that needs to be taken into account in the study of nano-immune interactions and safe use of NPs in biological systems. Moreover, our finding can be a new efficient strategy for activating inflammatory or antiinflammatory in medical treatment.
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