Morphological study of electrospun chitosan/poly(vinyl alcohol)/glycerol nanofibres for skin care applications

This study aimed to evaluate the influence of formulation and procedure parameters in obtaining thick and continuous chitosan/PVA/glycerol nanofibres to be applied in skin care. For that, the polymers were characterized by nuclear magnetic resonance, Fourier-transform infrared spectroscopy, and size-exclusion chromatography. After this, 96 chitosan/PVA/glycerol nanofibre scaffolds were prepared by electrospinning method, using factorial designs. The independent variables were crude and pure chitosan, 2 brands of PVA, 2 needle gauges, high and low polymer concentration, high and low glycerol concentration, and final solution with and without ultrafiltration. Morphological analysis was performed by scanning electron microscopy, atomic force microscopy, and confocal microscopy. The best sample (NF67) presented an average thickness of 268.3 nm, uniform distribution, and high yield. It was obtained at a 1:3.5 (crude chitosan: PVA with lower molecular weight, but more hydrolysed) ratio and lower glycerol concentration, suggesting that the degree of hydrolysis of the PVA is more important than its molecular weight for obtaining better quality nanofibres and that the glycerol also makes the electrospinning process difficult. Thus, it was possible to choose parameters that provide scaffolds that could be applied as a matrix extracellular-like material in wound healing.

Automated summary

This study evaluated the impact of formulation and procedure parameters on obtaining chitosan/PVA/glycerol nanofibres for skin care applications. By characterizing the polymers and using factorial designs to prepare scaffolds, it was found that the degree of hydrolysis of PVA is more crucial than its molecular weight for quality nanofibres, and glycerol can complicate the electrospinning process. The study identified parameters for scaffolds that could be used as extracellular matrix-like materials in wound healing.