4.7 Article

Lignin from Morinda citrifolia leaves: Physical and chemical characterization, in vitro evaluation of antioxidant, cytotoxic, antiparasitic and ultrastructural activities

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ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.11.013

关键词

Lignin; Phenolic macromolecule; Natural antioxidant; Antiparasitic activity

资金

  1. Brazilian agencies Foundation for Research Support of the State of Pernambuco-FACEPE [APQ-0498-4.03/19]
  2. National Council for Scientific and Technological Development-CNPq [303630/2017-5]

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The lignin extracted from Morinda citrifolia leaves showed low cytotoxicity and antioxidant activity, but exhibited inhibitory effects on Leishmania parasites. It is considered a promising macromolecule for use as an antiparasitic and antioxidant agent.
In this work, we investigated in vitro the antioxidant, cytotoxic and anti-leishmanial activities of a lignin extracted from the leaves of Morinda citrifolia. Initially, an analysis of the composition of the sheets was performed, then the lignin was obtained by alkaline delignification and characterized by different techniques: elemental analysis, FT-R, UV-vis, HSQC-NMR, thermal analysis, Py-GC/MS and by GPC. The results showed that the leaves had in their composition cellulose (31.29%), hemicellulose (25.01%), lignin (18.34%), extractives (14.39%) and ash (10.03%). The lignin extraction yield was 89.8%. The lignin obtained is of the GSH type with the following contents 79.39%, 13.58% and 7.03% respectively. Furthermore, it is low molecular weight and thermally stable. It had a phenolic content of 93.3 mg GAE/g and low antioxidant activity. In macrophage cytotoxicity assays, it presented a CC50 of 31.0 mu g/mL, showing less toxicity than amphotericin B. In assays against the promastigote forms of Leishmania amazonensis, lignin presented an IC50 of 29.56 mu g/mL, a less effective concentration than amphotericin B (IC50 = 0.14 mu g/mL). However, it was able to promote inhibition of the parasites, a fact confirmed by structural changes. These findings reinforce that M. citrifolia lignin is a promising macromolecule for use as an antiparasitic and antioxidant agent.

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