Production of polyhydroxyalkanoates by three novel species of Marinobacterium

Several species of novel marine bacteria from the genus Marinobacterium, including M. nitratireducens, M. sediminicola, and M. zhoushanense were found to be capable of producing polyhydroxyalkanoates (PHA) using sugars and volatile fatty acids (VFAs) as the carbon source. M. zhoushanense produced poly-3-hydroxybutytate (PHB) from sucrose, achieving a product titer and PHB content of 2.89 g/L and 64.05 wt%, respectively. By contrast, M. nitratireducens accumulated 3.38 g/L PHB and 66.80 wt% polymer content using butyrate as the substrate. A third species, M. sediminicola showed favorable tolerance to propionate, butyrate, and valerate. The use of 10 g/L valerate yielded 3.37 g/L poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), with a 3-hydroxyvalerate (3 HV) monomer content of 94.75 mol%. Moreover, M. sediminicola could be manipulated to produce PHBV with changeable polymer compositions by feeding different mixtures of VFAs. Our results indicate that M. sediminicola is a promising halophilic bacterium for the production of PHA.

Automated summary

Several marine bacteria from the genus Marinobacterium were found to produce polyhydroxyalkanoates (PHA) using sugars and volatile fatty acids (VFAs) as carbon sources. Different species showed varying abilities in producing PHAs, with M. zhoushanense producing poly-3-hydroxybutyrate (PHB) from sucrose, M. nitratireducens from butyrate, and M. sediminicola showing tolerance to different VFAs and ability to produce PHBV with changeable compositions.